The use of probes labeled with different reporter dyes allows the simultaneous detection and quantification of multiple target genes in a single (multiplex) reaction.
The most common type for real-time quantitative PCR is dual-labeled probes, often referred to as hydrolysis probes. These highly sensitive, bi-labeled fluorescent probes are designed to be sequence specific. We offer probes with an extensive range of fluorophores and a straightforward design that makes them suitable for use with most real-time qPCR instruments and multiplex analysis systems. Other popular qPCR probes include Molecular Beacons: structured probes that are highly sensitive, sequence-specific and used for sequence detection in real-time qPCR and in vitro studies. Our Scorpions™ probes are also highly sensitive and sequence-specific; these bi-labeled fluorescent probe/primer hybrids are designed for real-time quantitative PCR. The LightCycler fluorescent probes are designed for use with the Roche LightCycler instruments.
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